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Registros recuperados: 97
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Modulations of the interactions between pacific cupped oyster Crassostrea gigas and Vibrio according to bacterial virulence and to genetic and physiological status of the host : Methodology and first results ArchiMer
De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis.
Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and...
Tipo: Text Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf
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Morphological genetic markers for selection of broiler lines Rev. Bras. Ciênc. Avic.
Schmidt,GS; Coutinho,LL; Figueiredo,EAP de; Ledur,MC; Alves,HJ.
Two paternal broiler lines (LL and LLc, originated from the same stock in 1998) of Embrapa Suinos e Aves genetic program were used. Line LL had been selected for eight generations for rapid growth while LLc had been mated randomly, without selection, as a control line. In the last generation, 30 males and 240 females were used in hierarchical matings with pedigree control to determine sire effect. The same number of birds were used, but matings were at random for LLc. Eggs of both lines were incubated and embryos were collected in different development stages (40, 45, 50, 55 and 60 hours after setting) in order to determine somite number (NOS). The number of evaluated embryos were 1,967 and 498 for LL and LLc, respectively. This study aimed to determine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic; Heritability; Selection; Somite.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000300003
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Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf
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Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg) ArchiMer
Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe.
A novel method using 6-dimcthylaminopurinc (6-0MAP) has recently been demonstrated to be a good triploid inductor for bivalve molluscs. The effects of 6-DMAP concentration, timing of treatment after fertilization and treatment duration on survival and triploid induction of Crassostrea gigas (Thunberg) embryos were examined. An influence of sperm concentration was also detected and discussed. Survival to D-stage was inversely related to 6-DMAP concentration and the percentage of triploids was shown to be 6-DMAP dose dependent. Two optimal moments for treatment application were determined corresponding to extrusions of the first and second polar bodies. Optimal treatment of 450 µmol/l 6-DMAP beginning 15 min after fertilization over a 10 min period yielded...
Tipo: Text Palavras-chave: Genetic; Triploid induction; Crassostrea gigas; 6 DMAP; Triploid; Oysters.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/1994/publication-3198.pdf
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Overwiew on molecular tools in bivalve shellfish aquaculture ArchiMer
Boudry, Pierre; Saavedra, Carlos.
Dr. Boudry presented an overview of the current development of molecular tools in shellfish bivalves, focussing on the recent development in oysters, species to which the most efforts are dedicated. This review was prepared together with Dr. Saavedra (Mollusc Research Group, Torre de la Sal, Spain) within the Fish and Shellfish group of the EU Network of Excellence "Marine Genomics Europe". Searches in DNA sequence databank ("GenBank") show that 4% are microsatellite sequences, 23% are mitochondrial sequences and the remaining 73 % other nuclear sequences. Among these, 75% concern oyster (Crassostrea) species, 15% mussel (Mytilus) species and 10% scallop (Argopecten) species. Some of these sequences were targeted as candidate genes related to known...
Tipo: Text Palavras-chave: QTLs; Mitochondrial geneome; Crassostrea virginica; Crassostrea gigas; Crassostrea; Shellfish bivalves; Molecular tools; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3480.pdf
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Overwiew on selective breeding and genetic improvement in bivalve shellfish ArchiMer
Boudry, Pierre.
Dr. Boudry focussed his talk on oysters, which are the most important bivalve species produced world wide (more than 4 million tons). One of the main characteristic of bivalve aquaculture is that most of the juveniles ("seed or "spat") are collected from natural recruitment (e.g. 100% of the European mussel production). Hatchery propagation is used in the case of introduced species (e.g. Crassostrea gigas in USA) or limited natural recruitment. The most significant genetic improvement for the production of Pacific oyster (C. gigas) to date has been obtained through the breeding of triploids, especially since the development of tetraploids. Triploidy lead to highly reduced gametogenesis and, as a result better growth and survival. Many quantitative...
Tipo: Text Palavras-chave: Crassostrea gigas; Oyster; Genetic variability; Bivalve shellfish; Selective breeding; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3479.pdf
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Oyster population genetics : understanding natural populations and tracing introductions ArchiMer
Lapegue, Sylvie; Boudry, Pierre.
Oysters are among the most familiar of all marine invertebrate taxa. However our knowledge of oyster phylogeny and systematics is fragmentary. This is principally due to the plastic growth patterns of these animals, which result in a wide range of overlapping, ecophenotypic variants that greatly reduce the value of analysis based on shell morphology. Besides that, many intentional or accidental anthropogenic transfers have emphasised this situation. In several cases of misclassification or misidentification of oysters, DNA molecular data, have provided valuable new insights on the tracing of introductions or more generally on the phylogeographic relationships between oyster species. We will illustrate this issue with recent case studies from our lab on...
Tipo: Text Palavras-chave: Genetic; Ostreola stentina; Crassostrea honkongensis; Crassostrea gigas; Crassostrea angulata; Oysters; Population genetics.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3475.pdf
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Persistence of atrazine impact on aneuploidy in the Pacific oyster, Crassostrea gigas ArchiMer
Bouilly, Karine; Mccombie, Helen; Leitao, Alexandra; Lapegue, Sylvie.
Aneuploidy is the alteration of the normal diploid chromosome number. In the Pacific oyster, Crassostrea gigas, hypodiploid aneuploid cells have regularly been reported as has a negative correlation between this phenomenon and growth and evidence for a genetic basis. We previously demonstrated a positive relationship between a pollutant, atrazine, and aneuploidy in Crassostrea gigas adults and juveniles. To evaluate the persistence of this impact, the present study focused on a sample of the same juveniles exposed to different atrazine treatments (0.01 mg/l which represents a peak value found in a polluted environment and 0.1 mg/l) for three and a half months and evaluated them for aneuploidy after another two and a half months in non polluted conditions....
Tipo: Text Palavras-chave: Crassostrea gigas; Genetic; Pollutant persistence; Oyster culture; Marine molluscs; Herbicides; Cultured organisms; Chromosomes.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3344.pdf
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Pesquisas florestais da EMBRAPA na região Amazônica. Infoteca-e
SILVA, J. N. M.; CARPANEZZI, A. A.; COSTA, H. B. da; CARVALHO, J. O. P. de; LOPES, J. do C. A.; YARED, J. A. G.; MARQUES, L. C. T.; KANASHIRO, M.; VIANNA, N. G.; COSTA FILHO, P. P.; BRIENZA JUNIOR, S..
bitstream/item/50351/1/documentos130001.pdf
Tipo: Documentos (INFOTECA-E) Palavras-chave: Exploração; Melhoramento genético; Sistema agroflorestal; Brasil; Forest; Exploitation; Management; Genetic; Agroforesty system.; Ecologia; Floresta; Manejo; Pesquisa.; Amazonia; Ecology; Breeding; Research..
Ano: 1982 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/381407
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Phylogeography of mangrove oysters from the Southern Atlantic ocean : Crassostrea gasar and Crassostre rhizophorae ArchiMer
Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Thiriot-quievreux, Catherine; Heurtebise, Serge; Garcia, P.; Boudry, Pierre.
Mangrove oysters encompass several species and their taxomony is unclear. Among then, Crassostrea rhizophorae has been described along the Central and South American Atlantic coasts and Crassostrea gasar along African Atlantic coasts. Most taxonomic studies have so far been based on morphological criteria which are particularly susceptible to plasticity and new insight can be provided by molecular markers. The present work aims to provide information on the phylogeography of these two taxa.
Tipo: Text Palavras-chave: Genetic; Phylogeography; Crassostrea rhizophorae; Crassostrea gasar; Mangrove oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3496.pdf
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Physiological and molecular basis of gametogenesis in triploid Pacific oysters, Crassostrea gigas ArchiMer
Normand, Julien; Huvet, Arnaud; Fabioux, Caroline; Raguenes, Morgane; Boudry, Pierre.
Genetic improvement of the Pacific oyster, Crassostrea gigas, can be achieved through triploidisation, which leads to partially sterility, allowing higher growth and survival together with improved meat quality during the reproductive period. Most triploids are not fully sterile and the genetic and environmental factors involved in their partial fertility remained to be investigated. We examined gametogenesis by image analysis of histological cross sections in diploid (2n) and triploid oysters obtained either by crossing tetraploids and diploid broodstock (« natural » triploids: 3nn) or from second polar body retention (« chemical » triploids: 3nc). 5-month old triploid spat reared in a standard intensive nursery system only showed a reduction of 50% of...
Tipo: Text Palavras-chave: Gametogenesis; Triploid; Crassostrea gigas; Genetic; Oysters.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3327.pdf
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Population genetics of the European flat oyster (Ostrea edulis) : from larvae to populations ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, E.; Naciri-graven, Yamama; Ledu, Christophe; Mira, Sara; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
Nowadays, PCR-based DNA markers allow the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European flat oyster (Ostrea edulis) populations has aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Results were congruent between allozymes, microsatellites and mitochondrial DNA and support an isolation by distance model. High variability was observed in the breadth of diversity between populations using a mitochondrial marker, this highlights the small effective population sizes in some locations. A 10-fold quantitative difference was observed in Fst...
Tipo: Text Palavras-chave: Larvae; Reproduction; Genetic; Ostrea edulis; European flat oyster; Population genetics.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3493.pdf
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Primary forest management in Eastern Amazonia. Infoteca-e
EMBRAPA AMAZÔNIA ORIENTAL..
An evaluation for future research activities; Future actions: Dendrogene and jafa; Dendrogene; Nafa.
Tipo: Fôlder / Folheto / Cartilha (INFOTECA-E) Palavras-chave: Dendrogene; Nafa; Genetic; Amazon; Embrapa Amazônia Oriental; Brasil.; Amazonia..
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/995929
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Projet ANCRE-DMX2 : Indicateurs biologiques et écologiques pour une gestion durable des stocks de poissons DéMersauX profonds (100–700 m) d’intérêt halieutique à La Réunion ArchiMer
Roos, David; Aumond, Yoann; Huet, Jerome; Bruchon, Franck.
L'usage de la ligne à main mécanisée (moulinet électrique) s'est développé à La Réunion au cours des années 2000. Ce métier s’est développé à la suite d’une campagne de prospection des ressources halieutiques profondes (200-700m), menée conjointement par le CRPMEM et l’Ifremer en 2000. L’objectif de cette campagne par pêche au moulinet électrique visait à identifier de nouvelles ressources démersales à des fins de diversification pour la petite pêche artisanale côtière professionnelle réunionnaise. Ainsi, les premiers résultats de cette étude avaient mis en évidence l'existence de nouvelles espèces et des stocks encore peu exploités à l’époque. Leur exploitation par la pêche professionnelle mais aussi par la pêche plaisancière s’est dès lors développée...
Tipo: Text Palavras-chave: Poissons démersaux; Pêche profonde; La Réunion; Pêche artisanale côtière; Indicateurs biologiques et écologiques; Stock halieutique; Lutjanidae; Bramidae; Epinephelidae; Squalidae; Ligne à main mécanisée; Isotopes stables; Contenus stomacaux; Génétique; Sclérochronologie; Demersal fish; Deepwater fishery; Reunion island; Artisanal fishery; Bio-ecological indices; Fish stock; Lutjanidae; Bramidae; Epinephelidae; Squalidae; Electric reel fishing gear; Isotopic analyses; Stomach contents; Genetic; Sclerochronology.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00347/45812/45553.pdf
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Projet GERMON. Structure génétique et migration du thon Germon ArchiMer
Nikolic, Natacha; Puech, Alexis; Chouvelon, Tiphaine; Munschy, Catherine; Bodin, Nathalie; Brach-papa, Christophe; Potier, Michel; West, Wendy; Knoery, Joel; Zudaire, Iker; Dhurmeea, Zahirah; Degroote, Maxime; Cedras, Maria; Evano, Hugues; Bourjea, Jerome.
The GERMON project had several aims i) to understand the populations’ structure between albacore tuna southwest of the Indian Ocean and southeast Atlantic, ii) to determine the origin of juveniles observed in South Africa iii) to improve the understanding of the biology field (Size/Size and Size/Weight; reproduction and feeding area; trophic linkages) and iv) to initiate work on chemical analyzes and adaptation of the albacore species to its environment. These aims have been held and the results allow a better understanding of the management albacore. A general summary of results is presented in Part 9.
Tipo: Text Palavras-chave: Germon; Thunnus alalunga; Génétique; Stock; Structure; Connectivité; Chimie; Reproduction; Alimentation; Habitat; Albacore; Thunnus alalunga; Genetic; Stock; Structure; Connectivity; Chemical; Reproduction; Feeding behavior; Habitat.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00293/40461/39152.pdf
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Prospecção de cepas de agrobacterium spp. para a transformação genética de braquiária. Infoteca-e
CABRAL, G. B.; CARNEIRO, V. T. C.; SANTANA, C. G.; LEITE, J. A.; PIRES, M. V. V.; DUSI, D. M. A..
bitstream/CENARGEN/26633/1/bp115.pdf
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Agrobacterium spp; Prospecção; Transformação; Braquiária; Prospection; Genetic; Transformation.; Bactéria; Brachiaria; Genética; Gramínea..
Ano: 2005 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/187043
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QTL mapping for resistance to bonamiosis in the European flat oyster Ostrea edulis ArchiMer
Lallias, Delphine; Beaumont, Andy; Haley, Chris; Heurtebise, Serge; Boudry, Pierre; Lapegue, Sylvie.
A breeding programme has been undertaken by Ifremer to produce selected Ostrea edulis families which are resistant to Bonamia ostreae, a parasite which is the most significant constraint on aquaculture of this oyster. Using such families, we aimed to identify Quantitative Trait Loci (QTLs) of resistance/susceptibility to bonamiosis. We used an F2 family derived from a bi-parental cross between a wild oyster and an individual from one of the selected oyster families. A total of 550 F2 individuals from the family cohabited with wild oysters that were injected with the parasite. Mortality was checked daily over six months and heart smears were used on moribund or dying oysters to detect the presence and level of infection of the parasite. Ninety two F2...
Tipo: Text Palavras-chave: QTLs; Flat oyster; Ostrea edulis; Bonamia ostreae; Genetic; QTL.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-3726.pdf
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Quantitative trait loci mapping for meat quality traits in swine chromosome 6 Arq. Bras. Med. Vet. Zootec.
Pires,A.V.; Lopes,P.S.; Guimarães,S.E.F.; Guimarães,C.T.; Gomide,L.A.M.; Benevenuto Júnior,A.A.; Carmo,F.M.S..
The current study was carried out to perform QTL mapping on swine chromosome 6 (SSC6) associated to meat quality traits. The F2 population was produced by outbreed crossing using two native Brazilian breed Piau boars and 18 commercial sows. A total of 557 F2 animals were genotyped for 13 microsatellite markers. The traits evaluated on the F2 population were: pH measured 45 minutes and 24 hours post mortem (pH 45, pH24, respectively), drip loss (DL), cooking loss (CL), total loss (TL), intramuscular fat content (IMF), objective tenderness (OT), lightness (L), redness (A), yellowness (B), hue angle (h) and chrome (c). Data were analyzed by multiple regression developed for analysis of outbreed line crosses, using the QTL Express Software. Significant QTL...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QTL; Outbreed cross; Genetic; Molecular marker; Animal breeding.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500006
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Recent advances in EST sequencing in Crassostrea gigas: towards the sequencing of the Pacific oyster genome ArchiMer
Boudry, Pierre; Huvet, Arnaud; Lapegue, Sylvie; Sauvage, Christopher; Tanguy, A.; Moraga, D.; Goostrey, A.; Prunet, P.; Boulo, Viviane; Gueguen, Y.; Moal, Jeanne; Fleury, Elodie; Samain, Jean-francois; Mathieu, M.; Favrel, P..
Rationale for EST and genome sequencing of the Pacific oyster: • its membership of the Lophotrochozoa, an little studied branch of the Eukaryotes, • its worldwide aquacultural importance (4.2 million metric tons, worth 3.5 billion US dollars), • its high fecundity, with concomitantly high DNA polymorphism, • its use as a biosensor of coastal pollution. In 2004, an international community of biologists, the Oyster Genome Consortium (OGC) led by D. Hedgecock (University of Southern California) and uniting 70 participants from 10 countries, first presented the Pacific oyster Crassostrea gigas as a genome-sequencing candidate to Joint Genome Institute (JGI, USA) (Hedgecock et al., 2005). Besides this project (that remains to be accepted), several EST...
Tipo: Text Palavras-chave: Characteristics; Genome size; Genome; Crassostrea gigas; Oyster; Genetic.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3414.pdf
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Recomendacoes tecnicas para a cultura da soja na Regiao Central do Brasil. Infoteca-e
EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR)..
Solos e nutricao vegetal; Conservacao do solo; Correcao de solo e manutencao de fertilidade; Calagem; Qualidade do calcario e condicoes de uso; Correcao da acidez subsupercial; Adubacao em solos do Cerrado; Adubacao fosfatada; Adubacao potassica; Adubacao com micronutrientes; Adubacao foliar com macro e micronutrientes; Inoculacao; Plantas daninhas; Tecnologia de sementes; Estabelecimento de campo de semente; Colheita; Avaliacao; Manejo de pragas; Praticas culturais; Manejo do solo; Rotacao de culturas; Epoca de semeadura; Genetica e melhoramento; Fitopatologia.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Recomendacao tecnica; Adubacao fosfatada; Adubacao potassica; Planta daninha; Manejo de praga; Fitopatologia; Brasil; Soybean; Fertilizer; Seed; Inoculation; Weed; Genetic; Phytopathology; Pests management; Technical directions.; Adubação Foliar; Inoculação; Glycine Max; Genética; Melhoramento; Nutrição Vegetal; Pesquisa; Pratica Cultural; Semente; Soja; Solo.; Brazil; Crop management; Breeding; Plant nutrition; Soil..
Ano: 1991 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/447041
Registros recuperados: 97
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